| Host: |
Rabbit |
| Applications: |
ELISA/IP/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRKG1 (609-659) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Biotin |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.64-0.72 µg/µl |
| Dilution Range: |
WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
PRKG1 |
| Gene ID: |
5592 |
| Uniprot ID: |
KGP1_HUMAN |
| Immunogen Region: |
609-659 |
| Immunogen: |
Synthetic peptide taken within amino acid region 609-659 on human protein kinase G alpha subunit. |
| Tissue Specificity | Primarily expressed in lung and placenta. |
| Post Translational Modifications | Autophosphorylation increases kinase activity. 65 kDa monomer is produced by proteolytic cleavage. |
| Function | Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates IRAG1 and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation.decreasing contraction.controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle. |
| Protein Name | Cgmp-Dependent Protein Kinase 1Cgk 1Cgk1Cgmp-Dependent Protein Kinase ICgki |
| Database Links | Reactome: R-HSA-392517 Q13976-1Reactome: R-HSA-4086398Reactome: R-HSA-418457 Q13976-1 |
| Cellular Localisation | CytoplasmColocalized With Trpc7 In The Plasma Membrane |
| Alternative Antibody Names | Anti-Cgmp-Dependent Protein Kinase 1 antibodyAnti-Cgk 1 antibodyAnti-Cgk1 antibodyAnti-Cgmp-Dependent Protein Kinase I antibodyAnti-Cgki antibodyAnti-PRKG1 antibodyAnti-PRKG1B antibodyAnti-PRKGR1A antibodyAnti-PRKGR1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
