Anti-PRKAB1 antibody (50-150) [S7MR] (STJ11102047)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal AMPK Beta 1 (50-150) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKAB1 |
| Gene ID | 5564 |
| Uniprot ID | AAKB1_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | EEIKAPEKEEFLAWQHDLEV NDKAPAQARPTVFRWTGGGK EVYLSGSFNNWSKLPLTRSH NNFVAILDLPEGEHQYKFFV DGQWTHDPSEPIVTSQLGTV N |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human AMPK Beta 1 (Q9Y478). |
Additional Info
| Post Translational Modifications | Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Beta-1Ampk Subunit Beta-1Ampkb |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Beta-1 antibodyAnti-Ampk Subunit Beta-1 antibodyAnti-Ampkb antibodyAnti-PRKAB1 antibodyAnti-AMPK antibody |
Information sourced from Uniprot.org