Anti-PRDM14 antibody (1-260) (STJ27532)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRDM14 (1-260) antibody for use in WB, IHC-P, ELISA and ChIP in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g antibody for 10 Mu g-15 Mu g of Chromatin |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRDM14 |
| Gene ID | 63978 |
| Uniprot ID | PRD14_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MALPRPSEAVPQDKVCYPPE SSPQNLAAYYTPFPSYGHYR NSLATVEEDFQPFRQLEAAA SAAPAMPPFPFRMAPPLLSP GLGLQREPLYDLPWYSKLPP WYPIPHVPREVPPFLSSSHE YAGASSEDLGHQIIGGDNES GPCCGPDTLIPPPPADASLL PEGLRTSQLLPCSPSKQSED GPKPSNQEGKSPARFQFTEE DLHFVLYGVTPSLEHPASLH HAISGLLVPPDSSGSDSLP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRDM14 (NP_078780.1). |
Additional Info
| Tissue Specificity | Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level). |
| Function | Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTCTCCTAA-3'. |
| Protein Name | Pr Domain Zinc Finger Protein 14Pr Domain-Containing Protein 14 |
| Database Links | Reactome: R-HSA-452723 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pr Domain Zinc Finger Protein 14 antibodyAnti-Pr Domain-Containing Protein 14 antibodyAnti-PRDM14 antibody |
Information sourced from Uniprot.org