• Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody (STJ27532) and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunohistochemistry analysis of PRDM14 in paraffin-embedded rat testis using PRDM14 Rabbit polyclonal antibody (STJ27532) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from NCI-H125 cells, using PRDM14 Rabbit polyclonal antibody (STJ27532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using PRDM14 Rabbit polyclonal antibody (STJ27532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PRDM14 antibody (1-260) (STJ27532)

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STJ27532

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDM14 (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:20-1:100
ChIP 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDM14
Gene ID: 63978
Uniprot ID: PRD14_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRDM14 (NP_078780.1).
Immunogen Sequence: MALPRPSEAVPQDKVCYPPE SSPQNLAAYYTPFPSYGHYR NSLATVEEDFQPFRQLEAAA SAAPAMPPFPFRMAPPLLSP GLGLQREPLYDLPWYSKLPP WYPIPHVPREVPPFLSSSHE YAGASSEDLGHQIIGGDNES GPCCGPDTLIPPPPADASLL PEGLRTSQLLPCSPSKQSED GPKPSNQEGKSPARFQFTEE DLHFVLYGVTPSLEHPASLH HAISGLLVPPDSSGSDSLP
Tissue Specificity Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).
Function Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTCTCCTAA-3'.
Protein Name Pr Domain Zinc Finger Protein 14
Pr Domain-Containing Protein 14
Database Links Reactome: R-HSA-452723
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 14 antibody
Anti-Pr Domain-Containing Protein 14 antibody
Anti-PRDM14 antibody

Information sourced from Uniprot.org

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