• Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (STJ110659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g PPP4R1 antibody (STJ110659). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (STJ110659) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (STJ110659). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (STJ110659) at a dilition of 1:1000.
  • Western blot analysis of various lysates using PPP4R1 Rabbit polyclonal antibody (STJ110659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PPP4R1 antibody (324-647) (STJ110659)

SKU:
STJ110659

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP4R1 (324-647) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP4R1
Gene ID: 9989
Uniprot ID: PP4R1_HUMAN
Immunogen Region: 324-647
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
Immunogen Sequence: NPSSSGQYFKEESKSSEEMS VENKNRTRDQEAPEDVQVRP EDTPSDLSVSNSSVILENTM EDHAAEASGKPLGEISVPLD SSLLCTLSSESHQEAASNEN DKKPGNYKSMLRPEVGTTSQ DSALLDQELYNSFHFWRTPL PEIDLDIELEQNSGGKPSPE GPEEESEGPVPSSPNITMAT RKELEEMIENLEPHIDDPDV KAQVEVLSAALRASSLDAHE ETISIEKRSDLQDELDINE
Tissue Specificity Widely expressed with high expression in cultured mesangial cells. Isoform 1 and isoform 2 are expressed in renal tissues.
Function Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3.
Protein Name Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1 antibody
Anti-PPP4R1 antibody
Anti-MEG1 antibody
Anti-PP4R1 antibody

Information sourced from Uniprot.org

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