• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g PPP4C antibody (STJ25096). Western blot was performed from the immunoprecipitate using PPP4C antibody (STJ25096) at a dilution of 1:1000.
  • Immunohistochemistry analysis of PPP4C in paraffin-embedded human colon using PPP4C Rabbit polyclonal antibody (STJ25096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PPP4C in paraffin-embedded human lung cancer using PPP4C Rabbit polyclonal antibody (STJ25096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PPP4C in paraffin-embedded rat liver using PPP4C Rabbit polyclonal antibody (STJ25096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PPP4C Rabbit polyclonal antibody (STJ25096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PPP4C antibody (1-307) (STJ25096)

SKU:
STJ25096

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP4C (1-307) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP4C
Gene ID: 5531
Uniprot ID: PP4C_HUMAN
Immunogen Region: 1-307
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
Immunogen Sequence: MAEISDLDRQIEQLRRCELI KESEVKALCAKAREILVEES NVQRVDSPVTVCGDIHGQFY DLKELFRVGGDVPETNYLFM GDFVDRGFYSVETFLLLLAL KVRYPDRITLIRGNHESRQI TQVYGFYDECLRKYGSVTVW RYCTEIFDYLSLSAIIDGKI FCVHGGLSPSIQTLDQIRTI DRKQEVPHDGPMCDLLWSDP EDTTGWGVSPRGAGYLFGSD VVAQFNAANDIDMICRAHQ
Post Translational Modifications Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.
Function Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
Protein Name Serine/Threonine-Protein Phosphatase 4 Catalytic Subunit
Pp4c
Pp4
Protein Phosphatase X
Pp-X
Database Links Reactome: R-HSA-5693607
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 4 Catalytic Subunit antibody
Anti-Pp4c antibody
Anti-Pp4 antibody
Anti-Protein Phosphatase X antibody
Anti-Pp-X antibody
Anti-PPP4C antibody
Anti-PPP4 antibody
Anti-PPX antibody

Information sourced from Uniprot.org

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