| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (260-309 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP2CA |
| Gene ID: | 5515 |
| Uniprot ID: | PP2AA_HUMAN |
| Immunogen Region: | 260-309 aa |
| Specificity: | PP2A-C Alpha Polyclonal Antibody detects endogenous levels of PP2A-C Alpha protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human PP2A-alpha at the amino acid range 260-309 |
| Function | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC.promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3.promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3. Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome. |
| Protein Name | Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha IsoformPp2a-AlphaReplication Protein CRp-C |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-1295596Reactome: R-HSA-141444Reactome: R-HSA-163685Reactome: R-HSA-163767Reactome: R-HSA-180024Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-198753Reactome: R-HSA-202670Reactome: R-HSA-2465910Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2995383Reactome: R-HSA-389513Reactome: R-HSA-432142Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5663220Reactome: R-HSA-5673000Reactome: R-HSA-5675221Reactome: R-HSA-6804757Reactome: R-HSA-6811558Reactome: R-HSA-68877Reactome: R-HSA-69231Reactome: R-HSA-69273Reactome: R-HSA-9634600Reactome: R-HSA-9648025Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmNucleusChromosomeCentromereCytoskeletonSpindle PoleIn Prometaphase CellsBut Not In Anaphase CellsLocalizes At CentromeresDuring MitosisAlso Found At Spindle PolesCentromeric Localization Requires The Presence Of Sgo2 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibodyAnti-Pp2a-Alpha antibodyAnti-Replication Protein C antibodyAnti-Rp-C antibodyAnti-PPP2CA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
