• Immunofluorescence analysis of H9C2 cells using PP1 beta Rabbit polyclonal antibody (STJ115489) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PP1 beta Rabbit polyclonal antibody (STJ115489) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using PP1 beta Rabbit polyclonal antibody (STJ115489) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using PP1 beta Rabbit polyclonal antibody (STJ115489) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using PP1 beta Rabbit polyclonal antibody (STJ115489) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PP1 beta Rabbit polyclonal antibody (STJ115489) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PP1 beta antibody (STJ115489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-PPP1CB antibody (227-327) (STJ115489)

SKU:
STJ115489

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1CB (227-327) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1CB
Gene ID: 5500
Uniprot ID: PP1B_HUMAN
Immunogen Region: 227-327
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 227-327 of human PP1 beta (NP_002700.1).
Immunogen Sequence: GADVVSKFLNRHDLDLICRA HQVVEDGYEFFAKRQLVTLF SAPNYCGEFDNAGGMMSVDE TLMCSFQILKPSEKKAKYQY GGLNSGRPVTPPRTANPPKK R
Function Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
Protein Name Serine/Threonine-Protein Phosphatase Pp1-Beta Catalytic Subunit
Pp-1b
Ppp1cd
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-2173788
Reactome: R-HSA-2565942
Reactome: R-HSA-400253
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Reactome: R-HSA-5673000
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Highly Mobile In Cells And Can Be Relocalized Through Interaction With Targeting Subunits
In The Presence Of Ppp1r8 Relocalizes From The Nucleus To Nuclear Speckles
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase Pp1-Beta Catalytic Subunit antibody
Anti-Pp-1b antibody
Anti-Ppp1cd antibody
Anti-PPP1CB antibody

Information sourced from Uniprot.org

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