• Western blot analysis of various lysates, using PPP1CA antibody (STJ114342) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
  • Immunofluorescence analysis of PC-12 cells using PPP1CA rabbit polyclonal antibody (STJ114342) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PPP1CA antibody (230-330) (STJ114342)

SKU:
STJ114342

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1CA (230-330) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1CA
Gene ID: 5499
Uniprot ID: PP1A_HUMAN
Immunogen Region: 230-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-330 of human PPP1CA (NP_002699.1).
Immunogen Sequence: EVVAKFLHKHDLDLICRAHQ VVEDGYEFFAKRQLVTLFSA PNYCGEFDNAGAMMSVDETL MCSFQILKPADKNKGKYGQF SGLNPGGRPITPPRNSAKAK K
Post Translational Modifications Phosphorylated. Dephosphorylated at Thr-320 in the presence of ionizing radiation.
Function Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Dephosphorylates CENPA. Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy. (Microbial infection) Necessary for alphaviruses replication.
Protein Name Serine/Threonine-Protein Phosphatase Pp1-Alpha Catalytic Subunit
Pp-1a
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-180024
Reactome: R-HSA-2173788
Reactome: R-HSA-400253
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Primarily Nuclear And Largely Excluded From The Nucleolus
Highly Mobile In Cells And Can Be Relocalized Through Interaction With Targeting Subunits
Nom1 Plays A Role In Targeting This Protein To The Nucleolus
In The Presence Of Ppp1r8 Relocalizes From The Nucleus To Nuclear Speckles
Shuttles Toward The Cytosol During Infection With Veev
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase Pp1-Alpha Catalytic Subunit antibody
Anti-Pp-1a antibody
Anti-PPP1CA antibody
Anti-PPP1A antibody

Information sourced from Uniprot.org

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