![Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88716/404925/STJ110441_1__17740.1744890652.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and CYPOR knockout (KO) HeLa cells, using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88716/404926/STJ110441_2__78260.1744890652.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CYPOR knockout (KO) HeLa cells, using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Immunohistochemistry analysis of paraffin-embedded Human esophagus using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88716/404927/STJ110441_3__16694.1744890653.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human esophagus using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88716/404928/STJ110441_4__34491.1744890653.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HeLa cells using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88716/404929/STJ110441_5__68935.1744890654.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88716/404930/STJ110441_6__01253.1744890654.jpg?c=1 "Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] CYPOR Rabbit pAb (STJ110441) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/88716/404925/STJ110441_1__17740.1744890652.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (STJ110441) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
Anti-POR antibody (1-250) (STJ110441)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYPOR (1-250) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POR |
| Gene ID | 5447 |
| Uniprot ID | NCPR_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MINMGDSHVDTSSTVSEAVA EEVSLFSMTDMILFSLIVGL LTYWFLFRKKKEEVPEFTKI QTLTSSVRESSFVEKMKKTG RNIIVFYGSQTGTAEEFANR LSKDAHRYGMRGMSADPEEY DLADLSSLPEIDNALVVFCM ATYGEGDPTDNAQDFYDWLQ ETDVDLSGVKFAVFGLGNKT YEHFNAMGKYVDKRLEQLGA QRIFELGLGDDDGNLEEDFI TWREQFWPAVCEHFGVEAT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3). |
Additional Info
| Function | This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. |
| Protein Name | Nadph--Cytochrome P450 ReductaseCprP450r |
| Database Links | Reactome: R-HSA-211897 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Nadph--Cytochrome P450 Reductase antibodyAnti-Cpr antibodyAnti-P450r antibodyAnti-POR antibodyAnti-CYPOR antibody |
Information sourced from Uniprot.org