• Western blot analysis of extracts of various cell lines, using CYPOR antibody (STJ110441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of NIH-3T3 cells using CYPOR Polyclonal Antibody (STJ110441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from normal (control) and CYPOR knockout (KO) HeLa cells, using CYPOR antibody (STJ110441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using CYPOR Polyclonal Antibody (STJ110441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human esophagus using CYPOR antibody (STJ110441) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using CYPOR Polyclonal Antibody (STJ110441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CYPOR Polyclonal Antibody (STJ110441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using CYPOR antibody (STJ110441) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using CYPOR Polyclonal Antibody (STJ110441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from normal (control) and CYPOR knockout (KO) HeLa cells, using CYPOR antibody (STJ110441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using CYPOR Polyclonal Antibody (STJ110441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CYPOR antibody (STJ110441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-POR antibody (1-250) (STJ110441)

SKU:
STJ110441

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYPOR (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POR
Gene ID: 5447
Uniprot ID: NCPR_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).
Immunogen Sequence: MINMGDSHVDTSSTVSEAVA EEVSLFSMTDMILFSLIVGL LTYWFLFRKKKEEVPEFTKI QTLTSSVRESSFVEKMKKTG RNIIVFYGSQTGTAEEFANR LSKDAHRYGMRGMSADPEEY DLADLSSLPEIDNALVVFCM ATYGEGDPTDNAQDFYDWLQ ETDVDLSGVKFAVFGLGNKT YEHFNAMGKYVDKRLEQLGA QRIFELGLGDDDGNLEEDFI TWREQFWPAVCEHFGVEAT
Function This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Protein Name Nadph--Cytochrome P450 Reductase
Cpr
P450r
Database Links Reactome: R-HSA-211897
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Nadph--Cytochrome P450 Reductase antibody
Anti-Cpr antibody
Anti-P450r antibody
Anti-POR antibody
Anti-CYPOR antibody

Information sourced from Uniprot.org

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