• Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g POLR2H antibody (STJ115302). Western blot was performed from the immunoprecipitate using POLR2H antibody (STJ115302) at a dilution of 1:1000.
  • Western blot analysis of various lysates using POLR2H Rabbit polyclonal antibody (STJ115302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POLR2H antibody (1-150) (STJ115302)

SKU:
STJ115302

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLR2H (1-150) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR2H
Gene ID: 5437
Uniprot ID: RPAB3_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
Immunogen Sequence: MAGILFEDIFDVKDIDPEGK KFDRVSRLHCESESFKMDLI LDVNIQIYPVDLGDKFRLVI ASTLYEDGTLDDGEYNPTDD RPSRADQFEYVMYGKVYRIE GDETSTEAATRLSAYVSYGG LLMRLQGDANNLHGFEVDSR VYLLMKKLAF
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
Protein Name Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc3
Rna Polymerases I - Ii - And Iii Subunit Abc3
Dna-Directed Rna Polymerase Ii Subunit H
Dna-Directed Rna Polymerases I - Ii - And Iii 17.1 Kda Polypeptide
Rpb17
Rpb8 Homolog
Hrpb8
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-168325
Reactome: R-HSA-1834949
Reactome: R-HSA-203927
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5578749
Reactome: R-HSA-5601884
Reactome: R-HSA-5617472
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73780
Reactome: R-HSA-73863
Reactome: R-HSA-73980
Reactome: R-HSA-749476
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-76061
Reactome: R-HSA-76066
Reactome: R-HSA-76071
Reactome: R-HSA-77075
Reactome: R-HSA-8851708
Reactome: R-HSA-9018519
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc3 antibody
Anti-Rna Polymerases I - Ii - And Iii Subunit Abc3 antibody
Anti-Dna-Directed Rna Polymerase Ii Subunit H antibody
Anti-Dna-Directed Rna Polymerases I - Ii - And Iii 17.1 Kda Polypeptide antibody
Anti-Rpb17 antibody
Anti-Rpb8 Homolog antibody
Anti-Hrpb8 antibody
Anti-POLR2H antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance