Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g POLR2H antibody (STJ115302). Western blot was performed from the immunoprecipitate using POLR2H antibody (STJ115302) at a dilution of 1:1000.

Anti-POLR2H antibody (1-150) (STJ115302)

Name: Anti-POLR2H antibody (1-150) (STJ115302)
SKU: STJ115302
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

ClonalityPolyclonal

HostRabbit

ConjugationUnconjugated

IsotypeIgG

STJ115302

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General Information

Short Description	Rabbit polyclonal POLR2H (1-150) antibody for use in WB, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
Applications	WB/IP/ELISA
Host	Rabbit
Reactivity	Human/Mouse
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Clonality	Polyclonal
Isotype	IgG
Conjugation	Unconjugated
Concentration	Lot specific
Purification	Affinity purification
Dilution Range	WB:1:500-1:2000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation	PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene Symbol	POLR2H
Gene ID	5437
Uniprot ID	RPAB3_HUMAN
Immunogen Region	1-150
Immunogen Sequence	MAGILFEDIFDVKDIDPEGK KFDRVSRLHCESESFKMDLI LDVNIQIYPVDLGDKFRLVI ASTLYEDGTLDDGEYNPTDD RPSRADQFEYVMYGKVYRIE GDETSTEAATRLSAYVSYGG LLMRLQGDANNLHGFEVDSR VYLLMKKLAF
Specificity	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).

Additional Info

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
Protein Name	Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc3 Rna Polymerases I - Ii - And Iii Subunit Abc3 Dna-Directed Rna Polymerase Ii Subunit H Dna-Directed Rna Polymerases I - Ii - And Iii 17.1 Kda Polypeptide Rpb17 Rpb8 Homolog Hrpb8
Database Links	Reactome: R-HSA-112382 Reactome: R-HSA-113418 Reactome: R-HSA-167152 Reactome: R-HSA-167158 Reactome: R-HSA-167160 Reactome: R-HSA-167161 Reactome: R-HSA-167162 Reactome: R-HSA-167172 Reactome: R-HSA-167200 Reactome: R-HSA-167238 Reactome: R-HSA-167242 Reactome: R-HSA-167243 Reactome: R-HSA-167246 Reactome: R-HSA-167287 Reactome: R-HSA-167290 Reactome: R-HSA-168325 Reactome: R-HSA-1834949 Reactome: R-HSA-203927 Reactome: R-HSA-427413 Reactome: R-HSA-5250924 Reactome: R-HSA-5578749 Reactome: R-HSA-5601884 Reactome: R-HSA-5617472 Reactome: R-HSA-674695 Reactome: R-HSA-6781823 Reactome: R-HSA-6781827 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6796648 Reactome: R-HSA-6803529 Reactome: R-HSA-6807505 Reactome: R-HSA-72086 Reactome: R-HSA-72163 Reactome: R-HSA-72165 Reactome: R-HSA-72203 Reactome: R-HSA-73762 Reactome: R-HSA-73772 Reactome: R-HSA-73776 Reactome: R-HSA-73779 Reactome: R-HSA-73780 Reactome: R-HSA-73863 Reactome: R-HSA-73980 Reactome: R-HSA-749476 Reactome: R-HSA-75953 Reactome: R-HSA-75955 Reactome: R-HSA-76042 Reactome: R-HSA-76061 Reactome: R-HSA-76066 Reactome: R-HSA-76071 Reactome: R-HSA-77075 Reactome: R-HSA-8851708 Reactome: R-HSA-9018519 Reactome: R-HSA-9670095
Cellular Localisation	Nucleus Nucleolus
Alternative Antibody Names	Anti-Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc3 antibody Anti-Rna Polymerases I - Ii - And Iii Subunit Abc3 antibody Anti-Dna-Directed Rna Polymerase Ii Subunit H antibody Anti-Dna-Directed Rna Polymerases I - Ii - And Iii 17.1 Kda Polypeptide antibody Anti-Rpb17 antibody Anti-Rpb8 Homolog antibody Anti-Hrpb8 antibody Anti-POLR2H antibody

Information sourced from Uniprot.org

Citations

View product citations for antibody STJ115302 on CiteAb