• Immunohistochemistry analysis of paraffin-embedded human kidney cancer using PODXL Rabbit monoclonal antibody (STJ11103568) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PODXL antibody (STJ11103568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PODXL antibody (300-479) [S8MR] (STJ11103568)

SKU:
STJ11103568

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PODXL (300-479) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PODXL
Gene ID: 5420
Uniprot ID: PODXL_HUMAN
Immunogen Region: 300-479
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-479 of human PODXL (O00592).
Immunogen Sequence: SPATALRTPTLPETMSSSPT AASTTHRYPKTPSPTVAHES NWAKCEDLETQTQSEKQLVL NLTGNTLCAGGASDEKLISL ICRAVKATFNPAQDKCGIRL ASVPGSQTVVVKEITIHTKL PAKDVYERLKDKWDELKEAG VSDMKLGDQGPPEEAEDRFS MPLIITIVCMASFLLLVAAL
Tissue Specificity Glomerular epithelium cell (podocyte).
Post Translational Modifications N- and O-linked glycosylated. Sialoglycoprotein.
Function Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Protein Name Podocalyxin
Gctm-2 Antigen
Gp200
Podocalyxin-Like Protein 1
Pc
Pclp-1
Cellular Localisation Apical Cell Membrane
Cell Projection
Lamellipodium
Filopodium
Ruffle
Microvillus
Membrane Raft
Membrane
Single-Pass Type I Membrane Protein
In Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet Polarized
Colocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial Polarization
Colocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma Membrane
Its Association With The Membrane Raft Is Transient
Colocalizes With Actin Filaments
Ezr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli Form
Colocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium Cells
Forms Granular
Punctuated Pattern
Forming Patches
Preferentially Adopting A Polar Distribution
Located On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodia Establishing Contact With The Endothelial Cells
Colocalizes With The Submembrane Actin Of Lamellipodia
Particularly Associated With Ruffles
Colocalizes With Vinculin At Protrusions Of Cells
Colocalizes With Itgb1
Colocalizes With Pard3
Prkci
Exoc5
Ocln
Rab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis
Alternative Antibody Names Anti-Podocalyxin antibody
Anti-Gctm-2 Antigen antibody
Anti-Gp200 antibody
Anti-Podocalyxin-Like Protein 1 antibody
Anti-Pc antibody
Anti-Pclp-1 antibody
Anti-PODXL antibody
Anti-PCLP antibody
Anti-PCLP1 antibody

Information sourced from Uniprot.org

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