| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-PODXL (300-479) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S8MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PODXL |
| Gene ID: |
5420 |
| Uniprot ID: |
PODXL_HUMAN |
| Immunogen Region: |
300-479 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 300-479 of human PODXL (O00592). |
| Immunogen Sequence: |
SPATALRTPTLPETMSSSPT AASTTHRYPKTPSPTVAHES NWAKCEDLETQTQSEKQLVL NLTGNTLCAGGASDEKLISL ICRAVKATFNPAQDKCGIRL ASVPGSQTVVVKEITIHTKL PAKDVYERLKDKWDELKEAG VSDMKLGDQGPPEEAEDRFS MPLIITIVCMASFLLLVAAL |
| Tissue Specificity | Glomerular epithelium cell (podocyte). |
| Post Translational Modifications | N- and O-linked glycosylated. Sialoglycoprotein. |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. |
| Protein Name | PodocalyxinGctm-2 AntigenGp200Podocalyxin-Like Protein 1PcPclp-1 |
| Cellular Localisation | Apical Cell MembraneCell ProjectionLamellipodiumFilopodiumRuffleMicrovillusMembrane RaftMembraneSingle-Pass Type I Membrane ProteinIn Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet PolarizedColocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial PolarizationColocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma MembraneIts Association With The Membrane Raft Is TransientColocalizes With Actin FilamentsEzr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli FormColocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium CellsForms GranularPunctuated PatternForming PatchesPreferentially Adopting A Polar DistributionLocated On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodia Establishing Contact With The Endothelial CellsColocalizes With The Submembrane Actin Of LamellipodiaParticularly Associated With RufflesColocalizes With Vinculin At Protrusions Of CellsColocalizes With Itgb1Colocalizes With Pard3PrkciExoc5OclnRab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis |
| Alternative Antibody Names | Anti-Podocalyxin antibodyAnti-Gctm-2 Antigen antibodyAnti-Gp200 antibodyAnti-Podocalyxin-Like Protein 1 antibodyAnti-Pc antibodyAnti-Pclp-1 antibodyAnti-PODXL antibodyAnti-PCLP antibodyAnti-PCLP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
