| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PNMT |
| Gene ID: | 5409 |
| Uniprot ID: | PNMT_HUMAN |
| Immunogen Region: | 1-282 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human PNMT (NP_002677.1). |
| Immunogen Sequence: | MSGADRSPNAGAAPDSAPGQ AAVASAYQRFEPRAYLRNNY APPRGDLCNPNGVGPWKLRC LAQTFATGEVSGRTLIDIGS GPTVYQLLSACSHFEDITMT DFLEVNRQELGRWLQEEPGA FNWSMYSQHACLIEGKGECW QDKERQLRARVKRVLPIDVH QPQPLGAGSPAPLPADALVS AFCLEAVSPDLASFQRALDH ITTLLRPGGHLLLIGALEES WYLAGEARLTVVPVSEEEV |
| Function | Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor. Other substrates include phenylethanolamine and octopamine. Also methylates normetanephrine. |
| Protein Name | Phenylethanolamine N-MethyltransferasePnmtaseNoradrenaline N-Methyltransferase |
| Database Links | Reactome: R-HSA-209905 |
| Alternative Antibody Names | Anti-Phenylethanolamine N-Methyltransferase antibodyAnti-Pnmtase antibodyAnti-Noradrenaline N-Methyltransferase antibodyAnti-PNMT antibodyAnti-PENT antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89677/358170/STJ11100739_1__87167.1713122476.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
