• Western blot analysis of extracts of various cell lines, using PNKD antibody (STJ117854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using PNKD antibody (STJ117854) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using PNKD antibody (STJ117854) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PNKD antibody (STJ117854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PNKD antibody (1-270) (STJ117854)

SKU:
STJ117854

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNKD (1-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNKD
Gene ID: 25953
Uniprot ID: PNKD_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PNKD (NP_056303.3).
Immunogen Sequence: MAAVVAATALKGRGARNARV LRGILAGATANKASHNRTRA LQSHSSPEGKEEPEPLSPEL EYIPRKRGKNPMKAVGLAWY SLYTRTWLGYLFYRQQLRRA RNRYPKGHSKTQPRLFNGVK VLPIPVLSDNYSYLIIDTQA QLAVAVDPSDPRAVQASIEK EGVTLVAILCTHKHWDHSGG NRDLSRRHRDCRVYGSPQDG IPYLTHPLCHQDVVSVGRLQ IRALATPGHTQGHLVYLLD
Tissue Specificity Isoform 1 is only expressed in the brain. Isoform 2 is ubiquitously detected with highest expression in skeletal muscle and detected in myocardial myofibrils. Variant Val-7 and Val-9 are detected in the brain only.
Function Probable hydrolase that plays an aggravative role in the development of cardiac hypertrophy via activation of the NF-kappa-B signaling pathway.
Protein Name Probable Hydrolase Pnkd
Myofibrillogenesis Regulator 1
Mr-1
Paroxysmal Nonkinesiogenic Dyskinesia Protein
Trans-Activated By Hepatitis C Virus Core Protein 2
Cellular Localisation Isoform 1: Membrane
Peripheral Membrane Protein
Isoform 2: Cytoplasm
Nucleus
Isoform 3: Mitochondrion
Alternative Antibody Names Anti-Probable Hydrolase Pnkd antibody
Anti-Myofibrillogenesis Regulator 1 antibody
Anti-Mr-1 antibody
Anti-Paroxysmal Nonkinesiogenic Dyskinesia Protein antibody
Anti-Trans-Activated By Hepatitis C Virus Core Protein 2 antibody
Anti-PNKD antibody
Anti-KIAA1184 antibody
Anti-MR1 antibody
Anti-TAHCCP2 antibody
Anti-FKSG19 antibody
Anti-UNQ2491 antibody
Anti-PRO5778 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance