• Confocal imaging of C2C12 cells using PMP70/ABCD3 Rabbit monoclonal antibody (STJ11103558, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of HeLa cells using PMP70/ABCD3 Rabbit monoclonal antibody (STJ11103558, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using PMP70/ABCD3 Rabbit monoclonal antibody (STJ11103558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using PMP70/ABCD3 Rabbit monoclonal antibody (STJ11103558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PMP70/ABCD3 antibody (560-659) [S8MR] (STJ11103558)

SKU:
STJ11103558

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PMP70/ABCD3 (560-659) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCD3
Gene ID: 5825
Uniprot ID: ABCD3_HUMAN
Immunogen Region: 560-659
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 560-659 of human PMP70/ABCD3 (P28288).
Immunogen Sequence: GWDSVQDWMDVLSGGEKQRM AMARLFYHKPQFAILDECTS AVSVDVEGYIYSHCRKVGIT LFTVSHRKSLWKHHEYYLHM DGRGNYEFKQITEDTVEFGS
Post Translational Modifications Ubiquitinated by PEX2 during pexophagy in response to starvation, leading to its degradation.
Function Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation. Has fatty acyl-CoA thioesterase and ATPase activities. Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes. Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation.
Protein Name Atp-Binding Cassette Sub-Family D Member 3
70 Kda Peroxisomal Membrane Protein
Pmp70
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9603798
Cellular Localisation Peroxisome Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family D Member 3 antibody
Anti-70 Kda Peroxisomal Membrane Protein antibody
Anti-Pmp70 antibody
Anti-ABCD3 antibody
Anti-PMP70 antibody
Anti-PXMP1 antibody

Information sourced from Uniprot.org

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