• Immunofluorescence analysis of C6 cells using Phospholipid Phospholipid Scramblase 1 (PLSCR1) (PLSCR1) Rabbit monoclonal antibody (STJ11101867) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Phospholipid Phospholipid Scramblase 1 (PLSCR1) (PLSCR1) Rabbit monoclonal antibody (STJ11101867) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse lung, using Phospholipid Phospholipid Scramblase 1 (PLSCR1) (PLSCR1) Rabbit monoclonal antibody (STJ11101867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of HT-29 cells, using Phospholipid Phospholipid Scramblase 1 (PLSCR1) (PLSCR1) Rabbit monoclonal antibody (STJ11101867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PLSCR1 antibody (1-100) [S7MR] (STJ11101867)

SKU:
STJ11101867

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Scramblase 1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLSCR1
Gene ID: 5359
Uniprot ID: PLS1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Phospholipid Scramblase 1 (PLSCR1) (NP_066928.1).
Immunogen Sequence: MDKQNSQMNASHPETNLPVG YPPQYPPTAFQGPPGYSGYP GPQVSYPPPPAGHSGPGPAG FPVPNQPVYNQPVYNQPVGA AGVPWMPAPQPPLNCPPGLE
Tissue Specificity Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
Post Translational Modifications Phosphorylation at Thr-161 by PKC/PKCD increases its phospholipid scramblase activity during both cell stimulation and apoptosis. Phosphorylated by OXSR1 in the presence of RELT. Palmitoylation is required for its phospholipid scramblase activity. Palmitoylation regulates its localization to the cell membrane or the nucleus.trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus.
Function Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membrane resulting in collapse of the phospholipid asymmetry which leads to phosphatidylserine externalization on the cell surface. Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with TRPC5. Also exhibits magnesium-dependent nuclease activity against double-stranded DNA and RNA but not single-stranded DNA and can enhance DNA decatenation mediated by TOP2A. Negatively regulates FcR-mediated phagocytosis in differentiated macrophages. May contribute to cytokine-regulated cell proliferation and differentiation. May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. Inhibits the functions of viral transactivators, including human T-cell leukemia virus (HTLV)-1 protein Tax, human immunodeficiency virus (HIV)-1 Tat, human hepatitis B virus (HBV) HBx, Epstein-Barr virus (EBV) BZLF1 and human cytomegalovirus IE1 and IE2 proteins through direct interactions. Mediates also the inhibition of influenza virus infection by preventing nuclear import of the viral nucleoprotein/NP. Plays a crucial role as a defense factor against SARS-CoV-2 independently of its scramblase activity by directly targeting nascent viral vesicles to prevent virus-membrane fusion and the release of viral RNA into the host-cell cytosol. (Microbial infection) Acts as an attachment receptor for HCV.
Protein Name Phospholipid Scramblase 1
Pl Scramblase 1
Ca(2+-Dependent Phospholipid Scramblase 1
Erythrocyte Phospholipid Scramblase
Mg(2+-Dependent Nuclease
Mmtra1b
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Lipid-Anchor
Cytoplasmic Side
Nucleus
Cytoplasm
Perinuclear Region
Localizes To The Perinuclear Region In The Presence Of Relt
Palmitoylation Regulates Its Localization To The Cell Membrane Or The Nucleus
Trafficking To The Cell Membrane Is Dependent Upon Palmitoylation Whereas In The Absence Of Palmitoylation
Localizes To The Nucleus
Alternative Antibody Names Anti-Phospholipid Scramblase 1 antibody
Anti-Pl Scramblase 1 antibody
Anti-Ca(2+-Dependent Phospholipid Scramblase 1 antibody
Anti-Erythrocyte Phospholipid Scramblase antibody
Anti-Mg(2+-Dependent Nuclease antibody
Anti-Mmtra1b antibody
Anti-PLSCR1 antibody

Information sourced from Uniprot.org

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