• Western blot analysis of extracts of various cell lines, using tPA/tPA/PLAT rabbit monoclonal antibody (STJ11102017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of human placenta using tPA/PLAT rabbit monoclonal antibody (STJ11102017, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Confocal imaging of NIH/3T3 cells using tPA/PLAT rabbit monoclonal antibody (STJ11102017, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-PLAT antibody (463-562) [S7MR] (STJ11102017)

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STJ11102017

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PLAT (463-562) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLAT
Gene ID: 5327
Uniprot ID: TPA_HUMAN
Immunogen Region: 463-562
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 463-562 of human tPA/tPA/PLAT (P00750).
Immunogen Sequence: LKEAHVRLYPSSRCTSQHLL NRTVTDNMLCAGDTRSGGPQ ANLHDACQGDSGGPLVCLND GRMTLVGIISWGLGCGQKDV PGVYTKVTNYLDWIRDNMRP
Tissue Specificity Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
Post Translational Modifications The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin. N-glycosylation of Asn-152.the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. Characterization of O-linked glycan was studied in Bowes melanoma cell line.
Function Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
Protein Name Tissue-Type Plasminogen Activator
T-Pa
T-Plasminogen Activator
Tpa
Alteplase
Reteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B
Database Links Reactome: R-HSA-186797
Reactome: R-HSA-75205
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Tissue-Type Plasminogen Activator antibody
Anti-T-Pa antibody
Anti-T-Plasminogen Activator antibody
Anti-Tpa antibody
Anti-Alteplase antibody
Anti-Reteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B antibody
Anti-PLAT antibody

Information sourced from Uniprot.org

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