Anti-PLA2G2D antibody (21-145) (STJ28773)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PLA2G2D (21-145) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLA2G2D |
| Gene ID | 26279 |
| Uniprot ID | PA2GD_HUMAN |
| Immunogen Region | 21-145 |
| Immunogen Sequence | GILNLNKMVKQVTGKMPILS YWPYGCHCGLGGRGQPKDAT DWCCQTHDCCYDHLKTQGCS IYKDYYRYNFSQGNIHCSDK GSWCEQQLCACDKEVAFCLK RNLDTYQKRLRFYWRPHCRG QTPGC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 21-145 of human PLA2G2D (NP_036532.1). |
Additional Info
| Tissue Specificity | Highly expressed in pancreas and spleen and less abundantly in colon, thymus, placenta, small intestine, and prostate. |
| Function | Secretory calcium-dependent phospholipase A2 that primarily targets extracellular lipids, exerting anti-inflammatory and immunosuppressive functions. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. In draining lymph nodes, selectively hydrolyzes diacyl and alkenyl forms of phosphatidylethanolamines, releasing omega-3 polyunsaturated fatty acids (PUFAs) such as eicosapentaenoate and docosahexaenoate that are precursors of the anti-inflammatory lipid mediators, resolvins. During the resolution phase of acute inflammation drives docosahexaenoate-derived resolvin D1 synthesis, which suppresses dendritic cell activation and T-helper 1 immune response. May act in an autocrine and paracrine manner. Via a mechanism independent of its catalytic activity, promotes differentiation of regulatory T cells (Tregs) and participates in the maintenance of immune tolerance. May contribute to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. |
| Protein Name | Group Iid Secretory Phospholipase A2Giid Spla2Spla2-IidPla2iidPhosphatidylcholine 2-Acylhydrolase 2dSecretory-Type Pla - Stroma-Associated Homolog |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482801Reactome: R-HSA-1482839Reactome: R-HSA-1482922Reactome: R-HSA-1482925Reactome: R-HSA-1483166 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Group Iid Secretory Phospholipase A2 antibodyAnti-Giid Spla2 antibodyAnti-Spla2-Iid antibodyAnti-Pla2iid antibodyAnti-Phosphatidylcholine 2-Acylhydrolase 2d antibodyAnti-Secretory-Type Pla - Stroma-Associated Homolog antibodyAnti-PLA2G2D antibodyAnti-SPLASH antibody |
Information sourced from Uniprot.org