• Western blot analysis of various lysates using PITX3 Rabbit monoclonal antibody (STJ11102992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PITX3 antibody (1-100) [S2MR] (STJ11102992)

SKU:
STJ11102992

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PITX3 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PITX3
Gene ID: 5309
Uniprot ID: PITX3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PITX3 (O75364).
Immunogen Sequence: MEFGLLSEAEARSPALSLSD AGTPHPQLPEHGCKGQEHSD SEKASASLPGGSPEDGSLKK KQRRQRTHFTSQQLQELEAT FQRNRYPDMSTREEIAVWTN
Tissue Specificity Highly expressed in developing eye lens.
Function Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.
Protein Name Pituitary Homeobox 3
Homeobox Protein Pitx3
Paired-Like Homeodomain Transcription Factor 3
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pituitary Homeobox 3 antibody
Anti-Homeobox Protein Pitx3 antibody
Anti-Paired-Like Homeodomain Transcription Factor 3 antibody
Anti-PITX3 antibody
Anti-PTX3 antibody

Information sourced from Uniprot.org

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