Anti-PITX3 antibody (1-100) [S2MR] (STJ11102992)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal PITX3 (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PITX3 |
| Gene ID | 5309 |
| Uniprot ID | PITX3_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MEFGLLSEAEARSPALSLSD AGTPHPQLPEHGCKGQEHSD SEKASASLPGGSPEDGSLKK KQRRQRTHFTSQQLQELEAT FQRNRYPDMSTREEIAVWTN |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PITX3 (O75364). |
Additional Info
| Tissue Specificity | Highly expressed in developing eye lens. |
| Function | Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. |
| Protein Name | Pituitary Homeobox 3Homeobox Protein Pitx3Paired-Like Homeodomain Transcription Factor 3 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pituitary Homeobox 3 antibodyAnti-Homeobox Protein Pitx3 antibodyAnti-Paired-Like Homeodomain Transcription Factor 3 antibodyAnti-PITX3 antibodyAnti-PTX3 antibody |
Information sourced from Uniprot.org