Anti-PITX2 antibody (20-70 aa) (STJ13101676)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 20-70 of mouse Pituitary homeobox 2 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-PITX2 (20-70 aa) for use in IHC and WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Pitx2 |
| Gene ID | 18741 |
| Uniprot ID | PITX2_MOUSE |
| Immunogen | A synthetic peptide from aa region 20-70 of mouse Pituitary homeobox 2 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 20-70 aa |
| Specificity | Specific for PITX2. |
Additional Info
| Post Translational Modifications | Phosphorylated at Thr-90 by AKT2, but not AKT1. Phosphorylation impairs its association with a CCND1 mRNA-stabilizing complex, thus shortening the half-life of CCND1. |
| Function | May play a role in myoblast differentiation. When unphosphorylated, associates with an ELAVL1-containing complex, which stabilizes cyclin mRNA and ensuring cell proliferation. Phosphorylation by AKT2 impairs this association, leading to CCND1 mRNA destabilization and progression towards differentiation. Isoform Ptx2c: Involved in the establishment of left-right asymmetry in the developing embryo. |
| Protein Name | Pituitary Homeobox 2All1-Responsive Protein Arp1Brx1 HomeoproteinBicoid-Related Homeobox Protein 1Homeobox Protein Pitx2Orthodenticle-Like Homeobox 2Paired-Like Homeodomain Transcription Factor 2Solurshin |
| Database Links | |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Pituitary Homeobox 2 antibodyAnti-All1-Responsive Protein Arp1 antibodyAnti-Brx1 Homeoprotein antibodyAnti-Bicoid-Related Homeobox Protein 1 antibodyAnti-Homeobox Protein Pitx2 antibodyAnti-Orthodenticle-Like Homeobox 2 antibodyAnti-Paired-Like Homeodomain Transcription Factor 2 antibodyAnti-Solurshin antibodyAnti-Pitx2 antibodyAnti-Arp1 antibodyAnti-Brx1 antibodyAnti-Otlx2 antibodyAnti-Ptx2 antibodyAnti-Rgs antibodyAnti-Rieg antibody |
Information sourced from Uniprot.org