• Western blot analysis of lysates from HeLa cells, using PIK3CG Rabbit polyclonal antibody (STJ24995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIK3CG antibody (1-310) (STJ24995)

SKU:
STJ24995

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIK3CG (1-310) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3CG
Gene ID: 5294
Uniprot ID: PK3CG_HUMAN
Immunogen Region: 1-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human PIK3CG (NP_002640.2).
Immunogen Sequence: MELENYKQPVVLREDNCRRR RRMKPRSAAASLSSMELIPI EFVLPTSQRKCKSPETALLH VAGHGNVEQMKAQVWLRALE TSVAADFYHRLGPHHFLLLY QKKGQWYEIYDKYQVVQTLD CLRYWKATHRSPGQIHLVQR HPPSEESQAFQRQLTALIGY DVTDVSNVHDDELEFTRRGL VTPRMAEVASRDPKLYAMHP WVTSKPLPEYLWKKIANNCI FIVIHRSTTSQTIKVSPDD
Tissue Specificity Pancreas, skeletal muscle, liver and heart.
Post Translational Modifications Autophosphorylation at Ser-1101 has no effect on the phosphatidylinositol-4,5-bisphosphate 3-kinase activity.
Function Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation, activation, and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
Protein Name Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Gamma Isoform
Pi3-Kinase Subunit Gamma
Pi3k-Gamma
Pi3kgamma
Ptdins-3-Kinase Subunit Gamma
Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Gamma
Ptdins-3-Kinase Subunit P110-Gamma
P110gamma
Phosphoinositide-3-Kinase Catalytic Gamma Polypeptide
Serine/Threonine Protein Kinase Pik3cg
P120-Pi3k
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1660499
Reactome: R-HSA-392451
Reactome: R-HSA-9027276
Reactome: R-HSA-9680350
Cellular Localisation Cytoplasm
Cell Membrane
Alternative Antibody Names Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Gamma Isoform antibody
Anti-Pi3-Kinase Subunit Gamma antibody
Anti-Pi3k-Gamma antibody
Anti-Pi3kgamma antibody
Anti-Ptdins-3-Kinase Subunit Gamma antibody
Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Gamma antibody
Anti-Ptdins-3-Kinase Subunit P110-Gamma antibody
Anti-P110gamma antibody
Anti-Phosphoinositide-3-Kinase Catalytic Gamma Polypeptide antibody
Anti-Serine/Threonine Protein Kinase Pik3cg antibody
Anti-P120-Pi3k antibody
Anti-PIK3CG antibody

Information sourced from Uniprot.org

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