• STJ70320 (0. 3µg/ml) staining of HEK293 (A) , K562 (B) and (1ug/ml) LNCaP (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70320 (1µg/ml) staining of KNRK (A) , NIH3T3 (B and (0. 3ug/ml) Neuro2a (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70320 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PI31/PSMF1-Isoform 1 antibody (C-Term) (STJ70320)

SKU:
STJ70320-100

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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PI31/PSMF1-Isoform 1 (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PSMF1
Gene ID: 9491
Uniprot ID: PSMF1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_006805.2
Specificity: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
Immunogen Sequence: DHLPPPGYDDMYL
Protein Name Proteasome Inhibitor Pi31 Subunit
Hpi31
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1234176
Reactome: R-HSA-1236974
Reactome: R-HSA-1236978
Reactome: R-HSA-174084
Reactome: R-HSA-174113
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-180534
Reactome: R-HSA-180585
Reactome: R-HSA-187577
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-211733
Reactome: R-HSA-2467813
Reactome: R-HSA-2871837
Reactome: R-HSA-349425
Reactome: R-HSA-350562
Reactome: R-HSA-382556
Reactome: R-HSA-450408
Reactome: R-HSA-4608870
Reactome: R-HSA-4641257
Reactome: R-HSA-4641258
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5658442
Reactome: R-HSA-5668541
Reactome: R-HSA-5676590
Reactome: R-HSA-5678895
Reactome: R-HSA-5687128
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69481
Reactome: R-HSA-69601
Reactome: R-HSA-75815
Reactome: R-HSA-8852276
Reactome: R-HSA-8854050
Reactome: R-HSA-8939236
Reactome: R-HSA-8939902
Reactome: R-HSA-8941858
Reactome: R-HSA-8948751
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9020702
Reactome: R-HSA-9604323
Reactome: R-HSA-9755511
Reactome: R-HSA-9762114
Reactome: R-HSA-9824272
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Alternative Antibody Names Anti-Proteasome Inhibitor Pi31 Subunit antibody
Anti-Hpi31 antibody
Anti-PSMF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance