• Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using NIPA (Phospho-Ser354) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZC3HC1-Ser354 antibody (320-369 aa) (STJ90545)

SKU:
STJ90545

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Zinc finger C3HC-type protein 1-Ser354 (320-369 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZC3HC1
Gene ID: 51530
Uniprot ID: ZC3C1_HUMAN
Immunogen Region: 320-369 aa
Specificity: Phospho-NIPA (S354) Polyclonal Antibody detects endogenous levels of NIPA protein only when phosphorylated at S354.
Immunogen: The antiserum was produced against synthesized peptide derived from the human NIPA around the phosphorylation site of Ser354 at the amino acid range 320-369
Post Translational Modifications Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dependent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.
Function Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
Protein Name Zinc Finger C3hc-Type Protein 1
Nuclear-Interacting Partner Of Alk
Hnipa
Nuclear-Interacting Partner Of Anaplastic Lymphoma Kinase
Database Links Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger C3hc-Type Protein 1 antibody
Anti-Nuclear-Interacting Partner Of Alk antibody
Anti-Hnipa antibody
Anti-Nuclear-Interacting Partner Of Anaplastic Lymphoma Kinase antibody
Anti-ZC3HC1 antibody
Anti-NIPA antibody
Anti-HSPC216 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance