Anti-Phospho-WRN-Ser1141 antibody (1107-1156 aa) (STJ90561)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Werner Syndrome Helicase around the phosphorylation site of Ser1141 at the amino acid range 1107-1156
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Bifunctional 3 5 exonuclease/ATP-dependent helicase WRN-Ser1141 (1107-1156 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WRN |
| Gene ID | 7486 |
| Uniprot ID | WRN_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Werner Syndrome Helicase around the phosphorylation site of Ser1141 at the amino acid range 1107-1156 |
| Immunogen Region | 1107-1156 aa |
| Specificity | Phospho-WRN (S1141) Polyclonal Antibody detects endogenous levels of WRN protein only when phosphorylated at S1141. |
Additional Info
| Function | Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates. Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang. Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA. Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency.strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers.stably associates with foci elements generating binding sites for RP-A. Plays a role in double-strand break repair after gamma-irradiation. Unwinds some G-quadruplex DNA (d(CGG)n tracts).unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail. d(CGG)n tracts have a propensity to assemble into tetraplex structures.other G-rich substrates from a telomeric or IgG switch sequence are not unwound. Depletion leads to chromosomal breaks and genome instability. |
| Protein Name | Bifunctional 3'-5' Exonuclease/Atp-Dependent Helicase WrnDna Helicase - Recq-Like Type 3Recq Protein-Like 2Werner Syndrome Protein Includes - 3'-5' Exonuclease - Atp-Dependent HelicaseDna 3'-5' Helicase Wrn |
| Database Links | Reactome: R-HSA-174414Reactome: R-HSA-174437Reactome: R-HSA-3108214Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusNucleolusNucleoplasmChromosomeGamma-Irradiation Leads To Its Translocation From Nucleoli To Nucleoplasm And Pml Regulates The Irradiation-Induced Wrn RelocationLocalizes To Dna Damage Sites |
| Alternative Antibody Names | Anti-Bifunctional 3'-5' Exonuclease/Atp-Dependent Helicase Wrn antibodyAnti-Dna Helicase - Recq-Like Type 3 antibodyAnti-Recq Protein-Like 2 antibodyAnti-Werner Syndrome Protein Includes - 3'-5' Exonuclease - Atp-Dependent Helicase antibodyAnti-Dna 3'-5' Helicase Wrn antibodyAnti-WRN antibodyAnti-RECQ3 antibodyAnti-RECQL2 antibody |
Information sourced from Uniprot.org