Anti-Phospho-WAVE1-Tyr-125 antibody (STJA0041448)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-WAVE (Tyr-125) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-125 in human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in
General Information
| Short Description | Rabbit polyclonal anti-Phospho-WAVE1-Tyr-125 for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | WASF1 |
| Gene ID | 8936 |
| Uniprot ID | WASF1_HUMAN |
| Immunogen | Phospho-WAVE (Tyr-125) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-125 in human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in |
| Immunogen Sequence | Human |
| Specificity | This antibody was cross-adsorbed to phospho-WAVE (Tyr-150) and unphosphorylated WAVE (Tyr-125) peptides before affinity purification using phospho-WAVE (Tyr-125) peptide. In western blots, the antibody detects an 80 kDa* band corresponding to phospho |
Additional Info
| Tissue Specificity | Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12. |
| Function | Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Also involved in the regulation of mitochondrial dynamics. |
| Protein Name | Actin-Binding Protein Wasf1Protein Wave-1Verprolin Homology Domain-Containing Protein 1Wiskott-Aldrich Syndrome Protein Family Member 1Wasp Family Protein Member 1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-9013149Reactome: R-HSA-9013423Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonSynapseCell JunctionFocal AdhesionDot-Like Pattern In The CytoplasmConcentrated In Rac-Regulated Membrane-Ruffling AreasPartial Translocation To Focal Adhesion Sites Might Be Mediated By Interaction With Sorbs2In NeuronsColocalizes With Activated Ntrk2 After Bdnf Addition In Endocytic Sites Through The Association With Tmem108 |
| Alternative Antibody Names | Anti-Actin-Binding Protein Wasf1 antibodyAnti-Protein Wave-1 antibodyAnti-Verprolin Homology Domain-Containing Protein 1 antibodyAnti-Wiskott-Aldrich Syndrome Protein Family Member 1 antibodyAnti-Wasp Family Protein Member 1 antibodyAnti-WASF1 antibodyAnti-KIAA0269 antibodyAnti-SCAR1 antibodyAnti-WAVE1 antibody |
Information sourced from Uniprot.org