• Western blot analysis of lysates from K562 cells treated with Pervanadate, using p73 (Phospho-Tyr99) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of HeLa cells using Phospho-p73 (Y99) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using p73 (Phospho-Tyr99) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-p73 (Y99) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-TP73-Tyr99 antibody (66-115 aa) (STJ90378)

SKU:
STJ90378

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tumor protein p73-Tyr99 (66-115 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TP73
Gene ID: 7161
Uniprot ID: P73_HUMAN
Immunogen Region: 66-115 aa
Specificity: Phospho-p73 (Y99) Polyclonal Antibody detects endogenous levels of p73 protein only when phosphorylated at Y99.
Immunogen: The antiserum was produced against synthesized peptide derived from the human p73 around the phosphorylation site of Tyr99 at the amino acid range 66-115
Post Translational Modifications Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Polyubiquitinated by RCHY1/PIRH2.leading to its degradation by the proteasome.
Function Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression. It is an essential factor for the positive regulation of lung ciliated cell differentiation.
Protein Name Tumor Protein P73
P53-Like Transcription Factor
P53-Related Protein
Database Links Reactome: R-HSA-139915
Reactome: R-HSA-6803204
Reactome: R-HSA-6803205
Reactome: R-HSA-6803207
Reactome: R-HSA-6803211
Reactome: R-HSA-6804759
Reactome: R-HSA-8939236
Cellular Localisation Nucleus
Cytoplasm
Accumulates In The Nucleus In Response To Dna Damage
Alternative Antibody Names Anti-Tumor Protein P73 antibody
Anti-P53-Like Transcription Factor antibody
Anti-P53-Related Protein antibody
Anti-TP73 antibody
Anti-P73 antibody

Information sourced from Uniprot.org

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