Anti-Phospho-TP73-Tyr99 antibody (66-115 aa) (STJ90378)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human p73 around the phosphorylation site of Tyr99 at the amino acid range 66-115
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Tumor protein p73-Tyr99 (66-115 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TP73 |
| Gene ID | 7161 |
| Uniprot ID | P73_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human p73 around the phosphorylation site of Tyr99 at the amino acid range 66-115 |
| Immunogen Region | 66-115 aa |
| Specificity | Phospho-p73 (Y99) Polyclonal Antibody detects endogenous levels of p73 protein only when phosphorylated at Y99. |
Additional Info
| Post Translational Modifications | Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Polyubiquitinated by RCHY1/PIRH2.leading to its degradation by the proteasome. |
| Function | Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression. It is an essential factor for the positive regulation of lung ciliated cell differentiation. |
| Protein Name | Tumor Protein P73P53-Like Transcription FactorP53-Related Protein |
| Database Links | Reactome: R-HSA-139915Reactome: R-HSA-6803204Reactome: R-HSA-6803205Reactome: R-HSA-6803207Reactome: R-HSA-6803211Reactome: R-HSA-6804759Reactome: R-HSA-8939236 |
| Cellular Localisation | NucleusCytoplasmAccumulates In The Nucleus In Response To Dna Damage |
| Alternative Antibody Names | Anti-Tumor Protein P73 antibodyAnti-P53-Like Transcription Factor antibodyAnti-P53-Related Protein antibodyAnti-TP73 antibodyAnti-P73 antibody |
Information sourced from Uniprot.org