Anti-Phospho-TBC1D4-Ser318 antibody (STJ196242)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human AS160 (Ser318)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-TBC1 domain family member 4-Ser318 for use in WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TBC1D4 |
| Gene ID | 9882 |
| Uniprot ID | TBCD4_HUMAN |
| Immunogen | Synthesized phosho peptide around human AS160 (Ser318) |
| Specificity | This antibody detects endogenous levels of Human AS160 (phospho-Ser318) |
Additional Info
| Post Translational Modifications | Phosphorylated by AKT1.insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. |
| Function | May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. |
| Protein Name | Tbc1 Domain Family Member 4Akt Substrate Of 160 KdaAs160 |
| Database Links | Reactome: R-HSA-1445148 |
| Cellular Localisation | CytoplasmIsoform 2 Shows A Cytoplasmic Perinuclear Localization In A Myoblastic Cell Line In Resting And Insulin-Stimulated Cells |
| Alternative Antibody Names | Anti-Tbc1 Domain Family Member 4 antibodyAnti-Akt Substrate Of 160 Kda antibodyAnti-As160 antibodyAnti-TBC1D4 antibodyAnti-AS160 antibodyAnti-KIAA0603 antibody |
Information sourced from Uniprot.org