Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (STJ11102659) at 1:1000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. C6 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-PRKAA1/PRKAA2-T183/T172 antibody (STJ11102659)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102659
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General Information

Short DescriptionRabbit polyclonal Phospho-AMPKa1/AMPKa2-T183/T172 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPRKAA2
PRKAA1
Gene ID5563
5562
Uniprot IDAAPK2_HUMAN
AAPK1_HUMAN
Immunogen SequenceLRTSC
SpecificityA synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5).

Additional Info

Tissue Specificity
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-2 antibody
Anti-Ampk Subunit Alpha-2 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-PRKAA2 antibody
Anti-AMPK antibody
Anti-AMPK2 antibody
Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-1 antibody
Anti-Ampk Subunit Alpha-1 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-Tau-Protein Kinase Prkaa1 antibody
Anti-PRKAA1 antibody
Anti-AMPK1 antibody

Information sourced from Uniprot.org

Citations

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