Anti-Phospho-Polo-Like Kinase Kinase-Ser482/486/490 antibody (STJA0003750)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Polo-Like Kinase Kinase-Ser482/486/490 for use in WB in Rat, Xenopus, Human, Mouse and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Rat/Xenopus/Human/Mouse/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH. |
Additional Info
| Background | Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated 482 486 490 by phosphorylation at three sites (Ser, Ser and Ser ). Thus activation of PLK is thought to involve a kinase cascade 482, 486, 490 involving the phosphorylation of Ser in PLKK. |
Information sourced from Uniprot.org