| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Neutrophil cytosol factor 1-Ser345 (311-360 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NCF1 |
| Gene ID: | 653361 |
| Uniprot ID: | NCF1_HUMAN |
| Immunogen Region: | 311-360 aa |
| Specificity: | Phospho-p47-phox (S345) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S345. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human p47 phox around the phosphorylation site of Ser345 at the amino acid range 311-360 |
| Post Translational Modifications | Phosphorylated by PRKCD.phosphorylation induces activation of NCF1 and NADPH oxidase activity. |
| Function | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). |
| Protein Name | Neutrophil Cytosol Factor 1 Ncf-1 47 Kda Autosomal Chronic Granulomatous Disease Protein 47 Kda Neutrophil Oxidase Factor Ncf-47k Neutrophil Nadph Oxidase Factor 1 Nox Organizer 2 Nox-Organizing Protein 2 Sh3 And Px Domain-Containing Protein 1a P47-Phox |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-1236973 Reactome: R-HSA-3299685 Reactome: R-HSA-4420097 Reactome: R-HSA-5668599 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013423 |
| Cellular Localisation | Cytoplasm Cytosol Membrane Peripheral Membrane Protein Cytoplasmic Side |
| Alternative Antibody Names | Anti-Neutrophil Cytosol Factor 1 antibody Anti-Ncf-1 antibody Anti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibody Anti-47 Kda Neutrophil Oxidase Factor antibody Anti-Ncf-47k antibody Anti-Neutrophil Nadph Oxidase Factor 1 antibody Anti-Nox Organizer 2 antibody Anti-Nox-Organizing Protein 2 antibody Anti-Sh3 And Px Domain-Containing Protein 1a antibody Anti-P47-Phox antibody Anti-NCF1 antibody Anti-NOXO2 antibody Anti-SH3PXD1A antibody |
Information sourced from Uniprot.org