• Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293T/mouse-heart using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
  • Immunohistochemistry analysis of paraffin-embedded human brain, using MER/SKY (Phospho-Tyr749/681) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-MerTK/Tyro3 (Y749/681) Polyclonal Antibody diluted at 1：500

Anti-Phospho-MerTK/Tyro3-Tyr749/681 antibody (716-765 aa) (STJ90948)

SKU:
STJ90948

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase Mer and Tyrosine-protein kinase receptor TYRO3-Tyr749/681 (716-765 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MERTK
TYRO3
Gene ID: 10461
7301
Uniprot ID: MERTK_HUMAN
TYRO3_HUMAN
Immunogen Region: 716-765 aa
Specificity: Phospho-MerTK/Tyro3 (Y749/681) Polyclonal Antibody detects endogenous levels of MerTK/Tyro3 protein only when phosphorylated at Y749/681.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MER/SKY around the phosphorylation site of Tyr749/681 at the amino acid range 716-765
Post Translational Modifications Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2.
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Protein Name Tyrosine-Protein Kinase Mer
Proto-Oncogene C-Mer
Receptor Tyrosine Kinase Mertk
Database Links Reactome: R-HSA-202733
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Mer antibody
Anti-Proto-Oncogene C-Mer antibody
Anti-Receptor Tyrosine Kinase Mertk antibody
Anti-MERTK antibody
Anti-MER antibody

Information sourced from Uniprot.org

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