| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase kinase kinase 8-Thr290 (256-305 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:40000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP3K8 |
| Gene ID: | 1326 |
| Uniprot ID: | M3K8_HUMAN |
| Immunogen Region: | 256-305 aa |
| Specificity: | Phospho-Cot (T290) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at T290. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human COT around the phosphorylation site of Thr290 at the amino acid range 256-305 |
| Post Translational Modifications | Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290.the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB.the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages. |
| Function | Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 8 Cancer Osaka Thyroid Oncogene Proto-Oncogene C-Cot Serine/Threonine-Protein Kinase Cot Tumor Progression Locus 2 Tpl-2 |
| Database Links | Reactome: R-HSA-389357 Reactome: R-HSA-5684264 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 antibody Anti-Cancer Osaka Thyroid Oncogene antibody Anti-Proto-Oncogene C-Cot antibody Anti-Serine/Threonine-Protein Kinase Cot antibody Anti-Tumor Progression Locus 2 antibody Anti-Tpl-2 antibody Anti-MAP3K8 antibody Anti-COT antibody Anti-ESTF antibody |
Information sourced from Uniprot.org