| Host: |
Rabbit |
| Applications: |
WB/ELISA/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase kinase kinase 11-Thr277+Ser281 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-p 1:50-300ELISA 2000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP3K11 |
| Gene ID: |
4296 |
| Uniprot ID: |
M3K11_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human, Mouse, Rat MLK3 (Phospho Thr277+Ser281) |
| Immunogen: |
Synthesized peptide derived from human MLK3 (Phospho Thr277+Ser281) |
| Tissue Specificity | Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. |
| Post Translational Modifications | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42. |
| Function | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 11Mixed Lineage Kinase 3Src-Homology 3 Domain-Containing Proline-Rich Kinase |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-6802946Reactome: R-HSA-6802955Reactome: R-HSA-9013148Reactome: R-HSA-9013408Reactome: R-HSA-9013424Reactome: R-HSA-9649948 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeLocation Is Cell Cycle Dependent |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 11 antibodyAnti-Mixed Lineage Kinase 3 antibodyAnti-Src-Homology 3 Domain-Containing Proline-Rich Kinase antibodyAnti-MAP3K11 antibodyAnti-MLK3 antibodyAnti-PTK1 antibodyAnti-SPRK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
