• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MAP3K11-Thr277+Ser281 antibody (STJA0009834)

SKU:
STJA0009834

€57.50 - €282.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase kinase kinase 11-Thr277+Ser281 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP3K11
Gene ID: 4296
Uniprot ID: M3K11_HUMAN
Specificity: This antibody detects endogenous levels of Human, Mouse, Rat MLK3 (Phospho Thr277+Ser281)
Immunogen: Synthesized peptide derived from human MLK3 (Phospho Thr277+Ser281)
Tissue Specificity Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
Post Translational Modifications Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
Function Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 11
Mixed Lineage Kinase 3
Src-Homology 3 Domain-Containing Proline-Rich Kinase
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-6802946
Reactome: R-HSA-6802955
Reactome: R-HSA-9013148
Reactome: R-HSA-9013408
Reactome: R-HSA-9013424
Reactome: R-HSA-9649948
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Location Is Cell Cycle Dependent
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 11 antibody
Anti-Mixed Lineage Kinase 3 antibody
Anti-Src-Homology 3 Domain-Containing Proline-Rich Kinase antibody
Anti-MAP3K11 antibody
Anti-MLK3 antibody
Anti-PTK1 antibody
Anti-SPRK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance