| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase kinase kinase 1-Thr1402 (1368-1417 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP3K1 |
| Gene ID: | 4214 |
| Uniprot ID: | M3K1_HUMAN |
| Immunogen Region: | 1368-1417 aa |
| Specificity: | Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody detects endogenous levels of MEK Kinase-1 protein only when phosphorylated at T1402. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human MAP3K1 around the phosphorylation site of Thr1402 at the amino acid range 1368-1417 |
| Function | Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 1Mapk/Erk Kinase Kinase 1Mek Kinase 1Mekk 1 |
| Database Links | Reactome: R-HSA-166058Reactome: R-HSA-2871796Reactome: R-HSA-933542Reactome: R-HSA-975138Reactome: R-HSA-975871 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 antibodyAnti-Mapk/Erk Kinase Kinase 1 antibodyAnti-Mek Kinase 1 antibodyAnti-Mekk 1 antibodyAnti-MAP3K1 antibodyAnti-MAPKKK1 antibodyAnti-MEKK antibodyAnti-MEKK1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89087/357547/STJ11100014_1__92691.1713121666.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Anti-MAP3K1 antibody [EBS-T-008] (STJ16101082)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149798/302550/STJ16101082_1__26645.1646940701.jpg?c=1 "Anti-MAP3K1 antibody [EBS-T-008] (STJ16101082)")
