• Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S217/MAP2K2-S221 antibody (STJ22236) at dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Western blot analysis of lysates from HeLa cells using Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit polyclonal antibody (STJ22236) at 1:6000 dilution. HeLa cells were treated by PMA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime: 90s.

Anti-Phospho-MAP2K1/MAP2K2-S217/S221 antibody (STJ22236)

SKU:
STJ22236

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:7000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K1
MAP2K2
Gene ID: 5604
5605
Uniprot ID: MP2K1_HUMAN
MP2K2_HUMAN
Specificity: A synthetic phosphorylated peptide around S217 & S221 of human MAP2K1/MAP2K2MAP2K1 (NP_002746.1).
Immunogen Sequence: SMANS
Tissue Specificity
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 antibody
Anti-Map Kinase Kinase 1 antibody
Anti-Mapkk 1 antibody
Anti-Mkk1 antibody
Anti-Erk Activator Kinase 1 antibody
Anti-Mapk/Erk Kinase 1 antibody
Anti-Mek 1 antibody
Anti-MAP2K1 antibody
Anti-MEK1 antibody
Anti-PRKMK1 antibody
Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibody
Anti-Map Kinase Kinase 2 antibody
Anti-Mapkk 2 antibody
Anti-Erk Activator Kinase 2 antibody
Anti-Mapk/Erk Kinase 2 antibody
Anti-Mek 2 antibody
Anti-MAP2K2 antibody
Anti-MEK2 antibody
Anti-MKK2 antibody
Anti-PRKMK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance