• Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Antibody (STJ22166). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 rabbit polyclonal antibody (STJ22166) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 rabbit polyclonal antibody (STJ22166) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 rabbit polyclonal antibody (STJ22166) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-HDAC4/HDAC5/HDAC9-S246/S259/S220 antibody (STJ22166)

SKU:
STJ22166

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-HDAC4/HDAC5/HDAC9-S246/S259/S220 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC9
HDAC4
9734
9759
10014
HDAC9_HUMAN
HDAC4_HUMAN
HDAC5_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S246 of human HDAC4
Immunogen Sequence: Email for sequence
Tissue Specificity Q9UKV0 - Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
P56524 - Ubiquitous.
Q9UQL6 - Ubiquitous.
Q9UKV0 - Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
P56524 - Ubiquitous.
Q9UQL6 - Ubiquitous.
Alternative Antibody Names Anti-Histone Deacetylase 9 antibody
Anti-Hd9 antibody
Anti-Histone Deacetylase 7b antibody
Anti-Hd7 antibody
Anti-Hd7b antibody
Anti-Histone Deacetylase-Related Protein antibody
Anti-Mef2-Interacting Transcription Repressor Mitr antibody
Anti-HDAC9 antibody
Anti-HDAC7 antibody
Anti-HDAC7B antibody
Anti-HDRP antibody
Anti-KIAA0744 antibody
Anti-MITR antibody
Anti-Histone Deacetylase 4 antibody
Anti-Hd4 antibody
Anti-HDAC4 antibody
Anti-KIAA0288 antibody
Anti-Histone Deacetylase 5 antibody
Anti-Hd5 antibody
Anti-Antigen Ny-Co-9 antibody
Anti-HDAC5 antibody
Anti-KIAA0600 antibody
Anti-Histone Deacetylase 9 antibody
Anti-Hd9 antibody
Anti-Histone Deacetylase 7b antibody
Anti-Hd7 antibody
Anti-Hd7b antibody
Anti-Histone Deacetylase-Related Protein antibody
Anti-Mef2-Interacting Transcription Repressor Mitr antibody
Anti-HDAC9 antibody
Anti-HDAC7 antibody
Anti-HDAC7B antibody
Anti-HDRP antibody
Anti-KIAA0744 antibody
Anti-MITR antibody
Anti-Histone Deacetylase 4 antibody
Anti-Hd4 antibody
Anti-HDAC4 antibody
Anti-KIAA0288 antibody
Anti-Histone Deacetylase 5 antibody
Anti-Hd5 antibody
Anti-Antigen Ny-Co-9 antibody
Anti-HDAC5 antibody
Anti-KIAA0600 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance