• Immunohistochemistry analysis of paraffin-embedded human brain, using EPHA3/4/5 (Phospho-Tyr779/833) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EphA3/4/5-Tyr779/833 antibody (746-795 aa) (STJ91201)

SKU:
STJ91201

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Ephrin type-A receptor 3 and Ephrin type-A receptor 4 and Ephrin type-A receptor 5-Tyr779/833 (746-795 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHA3
EPHA5
2042
2044
2043
EPHA3_HUMAN
EPHA5_HUMAN
EPHA4_HUMAN
Immunogen Region: 746-795 aa
Specificity: Phospho-EphA3/4/5 (Y779/833) Polyclonal Antibody detects endogenous levels of EphA3/4/5 protein only when phosphorylated at Y779/833.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EPHA3/4/5 around the phosphorylation site of Tyr779/833 at the amino acid range 746-795
Post Translational Modifications Autophosphorylates upon activation by EFNA5. Phosphorylation on Tyr-602 mediates interaction with NCK1. Dephosphorylated by PTPN1.
Function Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Protein Name Ephrin Type-A Receptor 3
Eph-Like Kinase 4
Ek4
Hek4
Hek
Human Embryo Kinase
Tyrosine-Protein Kinase Tyro4
Tyrosine-Protein Kinase Receptor Etk1
Eph-Like Tyrosine Kinase 1
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-3928663
Reactome: R-HSA-3928665
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Ephrin Type-A Receptor 3 antibody
Anti-Eph-Like Kinase 4 antibody
Anti-Ek4 antibody
Anti-Hek4 antibody
Anti-Hek antibody
Anti-Human Embryo Kinase antibody
Anti-Tyrosine-Protein Kinase Tyro4 antibody
Anti-Tyrosine-Protein Kinase Receptor Etk1 antibody
Anti-Eph-Like Tyrosine Kinase 1 antibody
Anti-EPHA3 antibody
Anti-ETK antibody
Anti-ETK1 antibody
Anti-HEK antibody
Anti-TYRO4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance