Anti-Phospho-EIF4B-Ser422 antibody (388-437 aa) (STJ90440)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human eIF4B around the phosphorylation site of Ser422 at the amino acid range 388-437
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Eukaryotic translation initiation factor 4B-Ser422 (388-437 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF4B |
| Gene ID | 1975 |
| Uniprot ID | IF4B_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human eIF4B around the phosphorylation site of Ser422 at the amino acid range 388-437 |
| Immunogen Region | 388-437 aa |
| Specificity | Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422. |
Additional Info
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Protein Name | Eukaryotic Translation Initiation Factor 4bEif-4b |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-166208Reactome: R-HSA-429947Reactome: R-HSA-72649Reactome: R-HSA-72662Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-9820841Reactome: R-HSA-9820865 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4b antibodyAnti-Eif-4b antibodyAnti-EIF4B antibody |
Information sourced from Uniprot.org