| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Bovine/Canine/Mouse/Non-Human Primates/Rat/Sheep/Xenopus |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Che-1/AATF-Ser477 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was antigen affinity purified from pooled serum. |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AATF |
| Gene ID: |
26574 |
| Uniprot ID: |
AATF_HUMAN |
| Immunogen: |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH. |
| Tissue Specificity | Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus. |
| Post Translational Modifications | Hyperphosphorylated during the G1/S phase transition. |
| Function | Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. |
| Protein Name | Protein AatfApoptosis-Antagonizing Transcription FactorRb-Binding Protein Che-1 |
| Database Links | Reactome: R-HSA-193648 |
| Cellular Localisation | NucleusNucleolus |
| Alternative Antibody Names | Anti-Protein Aatf antibodyAnti-Apoptosis-Antagonizing Transcription Factor antibodyAnti-Rb-Binding Protein Che-1 antibodyAnti-AATF antibodyAnti-CHE1 antibodyAnti-DED antibodyAnti-HSPC277 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
