Host: | Rabbit |
Applications: | WB/ICC |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Phospho-N-Cadherin-Tyr-820 is suitable for use in Western Blot and Immunocytochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Purification: | Antigen Affinity Purified |
Dilution Range: | WB 1:1000 IHC ICC 1:200 IP |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | CDH2 |
Gene ID: | 1000 |
Uniprot ID: | CADH2_HUMAN |
Specificity: | This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-860) and unphosphorylated N-cadherin (Tyr-820) peptides before affinity purification using phospho-N-cadherin (Tyr-820) peptide. The purified antibody detects a 130 kDa* band corresponding t |
Immunogen: | Phospho-N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from t |
Post Translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN. |
Function | Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
Protein Name | Cadherin-2 Cdw325 Neural Cadherin N-Cadherin Cd Antigen Cd325 |
Database Links | Reactome: R-HSA-381426 Reactome: R-HSA-418990 Reactome: R-HSA-525793 Reactome: R-HSA-8957275 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Sarcolemma Cell Junction Cell Surface Desmosome Adherens Junction Colocalizes With Tmem65 At The Intercalated Disk In Cardiomyocytes Colocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes |
Alternative Antibody Names | Anti-Cadherin-2 antibody Anti-Cdw325 antibody Anti-Neural Cadherin antibody Anti-N-Cadherin antibody Anti-Cd Antigen Cd325 antibody Anti-CDH2 antibody Anti-CDHN antibody Anti-NCAD antibody |
Information sourced from Uniprot.org