• Western blot analysis of extracts of HeLa cells, using Phospho-HP1 gamma/CBX3-S83 polyclonal antibody (STJ117899) at 1:1000 dilution or HP1 gamma/CBX3 antibody (A2248). HeLa cells were treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HP1 gamma/CBX3-S83 Rabbit polyclonal antibody (STJ117899) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HP1 gamma/CBX3-S83 antibody (STJ117899) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-HP1 gamma/CBX3-S83 polyclonal antibody (STJ117899) at 1:1000 dilution or HP1 gamma/CBX3 antibody (A2248). HeLa cells were treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CBX3-S83 antibody (STJ117899)

SKU:
STJ117899

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CBX3-S83 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX3
Gene ID: 11335
Uniprot ID: CBX3_HUMAN
Immunogen: A synthetic phosphorylated peptide around S83 of human HP1 gamma/CBX3 (NP_009207.2).
Immunogen Sequence: RKSLS
Post Translational Modifications Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Function Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).
Protein Name Chromobox Protein Homolog 3
Hech
Heterochromatin Protein 1 Homolog Gamma
Hp1 Gamma
Modifier 2 Protein
Database Links Reactome: R-HSA-427389
Reactome: R-HSA-73772
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Associates With Euchromatin And Is Largely Excluded From Constitutive Heterochromatin
May Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential)
Alternative Antibody Names Anti-Chromobox Protein Homolog 3 antibody
Anti-Hech antibody
Anti-Heterochromatin Protein 1 Homolog Gamma antibody
Anti-Hp1 Gamma antibody
Anti-Modifier 2 Protein antibody
Anti-CBX3 antibody

Information sourced from Uniprot.org

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