Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Phospho-Breast cancer anti-estrogen resistance protein 1-Tyr410 (376-425 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | BCAR1 |
Gene ID: | 9564 |
Uniprot ID: | BCAR1_HUMAN |
Immunogen Region: | 376-425 aa |
Specificity: | Phospho-p130 Cas (Y410) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y410. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human p130 Cas around the phosphorylation site of Tyr410 at the amino acid range 376-425 |
Post Translational Modifications | PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif.phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner.phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway. |
Function | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling. |
Protein Name | Breast Cancer Anti-Estrogen Resistance Protein 1 Crk-Associated Substrate Cas Scaffolding Protein Family Member 1 P130cas |
Database Links | Reactome: R-HSA-186763 Reactome: R-HSA-372708 Reactome: R-HSA-4420097 Reactome: R-HSA-8849471 |
Cellular Localisation | Cell Junction Focal Adhesion Cytoplasm Cell Projection Axon Unphosphorylated Form Localizes In The Cytoplasm Localizes To Focal Adhesion Sites Following Integrin Engagement |
Alternative Antibody Names | Anti-Breast Cancer Anti-Estrogen Resistance Protein 1 antibody Anti-Crk-Associated Substrate antibody Anti-Cas Scaffolding Protein Family Member 1 antibody Anti-P130cas antibody Anti-BCAR1 antibody Anti-CAS antibody Anti-CASS1 antibody Anti-CRKAS antibody |
Information sourced from Uniprot.org