Anti-Phospho-BCAR1-Tyr410 antibody (376-425 aa) (STJ90765)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human p130 Cas around the phosphorylation site of Tyr410 at the amino acid range 376-425
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Breast cancer anti-estrogen resistance protein 1-Tyr410 (376-425 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCAR1 |
| Gene ID | 9564 |
| Uniprot ID | BCAR1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human p130 Cas around the phosphorylation site of Tyr410 at the amino acid range 376-425 |
| Immunogen Region | 376-425 aa |
| Specificity | Phospho-p130 Cas (Y410) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y410. |
Additional Info
| Post Translational Modifications | PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif.phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner.phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway. |
| Function | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling. |
| Protein Name | Breast Cancer Anti-Estrogen Resistance Protein 1Crk-Associated SubstrateCas Scaffolding Protein Family Member 1P130cas |
| Database Links | Reactome: R-HSA-186763Reactome: R-HSA-372708Reactome: R-HSA-4420097Reactome: R-HSA-8849471 |
| Cellular Localisation | Cell JunctionFocal AdhesionCytoplasmCell ProjectionAxonUnphosphorylated Form Localizes In The CytoplasmLocalizes To Focal Adhesion Sites Following Integrin Engagement |
| Alternative Antibody Names | Anti-Breast Cancer Anti-Estrogen Resistance Protein 1 antibodyAnti-Crk-Associated Substrate antibodyAnti-Cas Scaffolding Protein Family Member 1 antibodyAnti-P130cas antibodyAnti-BCAR1 antibodyAnti-CAS antibodyAnti-CASS1 antibodyAnti-CRKAS antibody |
Information sourced from Uniprot.org