| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Rho GTPase-activating protein 35-Tyr1105 (1071-1120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ARHGAP35 |
| Gene ID: |
2909 |
| Uniprot ID: |
RHG35_HUMAN |
| Immunogen Region: |
1071-1120 aa |
| Specificity: |
Phospho-GRF-1 (Y1105) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1105. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human GRF-1 around the phosphorylation site of Tyr1105 at the amino acid range 1071-1120 |
| Post Translational Modifications | Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I. Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids. In brain, phosphorylated by FYN and SRC. During focal adhesion formation, phosphorylated by MAPK1 and MAPK3 at the C-terminal region, probably at Ser-1451, Ser-1476, Thr-1480 and Ser-1483. Phosphorylation by MAPK1 and MAPK3 inhibits GAP function and localizes ARGHAP35 away from newly forming focal adhesions and stress fibers in cells spreading on fibronectin. Phosphorylation at Ser-1476 and Thr-1480 by GSK3B requires priming by MAPK and inhibits RhoGAP activity and modulates polarized cell migration. |
| Function | Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis. Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation. Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation. During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth. Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'.this function is however unclear and would need additional experimental evidences. |
| Protein Name | Rho Gtpase-Activating Protein 35Glucocorticoid Receptor Dna-Binding Factor 1Glucocorticoid Receptor Repression Factor 1Grf-1Rho Gap P190aP190-A |
| Database Links | Reactome: R-HSA-416550Reactome: R-HSA-8849471Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013406Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013423Reactome: R-HSA-9696264Reactome: R-HSA-9696270Reactome: R-HSA-9696273 |
| Cellular Localisation | CytoplasmCytoskeletonCilium Basal BodyNucleusCell MembraneIn Response To Integrins And Sdc4 And Upon Phosphorylation By PkcRelocalizes From The Cytoplasm To Regions Of Plasma Membrane Ruffling Where It Colocalizes With Polymerized Actin |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 35 antibodyAnti-Glucocorticoid Receptor Dna-Binding Factor 1 antibodyAnti-Glucocorticoid Receptor Repression Factor 1 antibodyAnti-Grf-1 antibodyAnti-Rho Gap P190a antibodyAnti-P190-A antibodyAnti-ARHGAP35 antibodyAnti-GRF1 antibodyAnti-GRLF1 antibodyAnti-KIAA1722 antibodyAnti-P190A antibodyAnti-p190ARHOGAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
