| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Rho GTPase-activating protein 35-Tyr1087 is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ARHGAP35 |
| Gene ID: | 2909 |
| Uniprot ID: | RHG35_HUMAN |
| Specificity: | Phospho-GRF-1 (Y1087) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1087. |
| Immunogen: | Synthesized phospho-peptide around the phosphorylation site of human GRF-1 (phospho Tyr1087) |
| Post Translational Modifications | Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I. Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids. In brain, phosphorylated by FYN and SRC. During focal adhesion formation, phosphorylated by MAPK1 and MAPK3 at the C-terminal region, probably at Ser-1451, Ser-1476, Thr-1480 and Ser-1483. Phosphorylation by MAPK1 and MAPK3 inhibits GAP function and localizes ARGHAP35 away from newly forming focal adhesions and stress fibers in cells spreading on fibronectin. Phosphorylation at Ser-1476 and Thr-1480 by GSK3B requires priming by MAPK and inhibits RhoGAP activity and modulates polarized cell migration. |
| Function | Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis. Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation. Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation. During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth. Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'.this function is however unclear and would need additional experimental evidences. |
| Protein Name | Rho Gtpase-Activating Protein 35 Glucocorticoid Receptor Dna-Binding Factor 1 Glucocorticoid Receptor Repression Factor 1 Grf-1 Rho Gap P190a P190-A |
| Database Links | Reactome: R-HSA-416550 Reactome: R-HSA-8849471 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013405 Reactome: R-HSA-9013406 Reactome: R-HSA-9013408 Reactome: R-HSA-9013409 Reactome: R-HSA-9013423 Reactome: R-HSA-9696264 Reactome: R-HSA-9696270 Reactome: R-HSA-9696273 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cilium Basal Body Nucleus Cell Membrane In Response To Integrins And Sdc4 And Upon Phosphorylation By Pkc Relocalizes From The Cytoplasm To Regions Of Plasma Membrane Ruffling Where It Colocalizes With Polymerized Actin |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 35 antibody Anti-Glucocorticoid Receptor Dna-Binding Factor 1 antibody Anti-Glucocorticoid Receptor Repression Factor 1 antibody Anti-Grf-1 antibody Anti-Rho Gap P190a antibody Anti-P190-A antibody Anti-ARHGAP35 antibody Anti-GRF1 antibody Anti-GRLF1 antibody Anti-KIAA1722 antibody Anti-P190A antibody Anti-p190ARHOGAP antibody |
Information sourced from Uniprot.org